Identification of Cryptosporidium parvum in Stool Specimen Using Different Diagnostic Methods in Wasit Province/Iraq

The present study aimed to investigate the presence and prevalence of Cryptosporidium amongimmunocompromised patients in Wasit Hospitals health centers determine best method for itsdiagnosis. started from 1st August end November 2020. One hundred forty stoolsamples were collected immunocompromised both genders who attended hospitaland at province. Stool samples inspected by modified acid-fast stain as a standardmethod, enzyme linked immunosorbent assay (ELISA) Molecular Nested PCR method. Modified Ziehl-Neelsen technique revealed 46 (33%) positive cases oocysts, 85 males 55 females,highly significant relationship was found between different age groups infectionwith highly difference rural urban area. was57.14% using nested 38.46% ELISA comparison with MZN It is concluded thatcryptosporidiosis be endemic Province first time mostreliable its diagnosis.